Abstract

Mitochondrial genomes (also known as mitogenomes) serve as valuable molecular markers and have found widespread applications in molecular biology and ecology. There is abundant sequence variation in vertebrate mitogenomes, and occasionally, they exhibit gene rearrangements. In this study, two Chinese endemic Rana species, Rana jiemuxiensis and Rana hanluica, were sequenced and analyzed to obtain their complete mitogenomes. The two species were sympatrically distributed in the Zhangjiajie National Forest Park, in Wulingyuan District, Zhangjiajie City, Hunan Province, China. The mitogenome of R. jiemuxiensis was 17,506 bp, while that of R. hanluica was 17,505 bp, each comprising 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), two ribosomal RNA genes (rRNAs), and a non-coding control region (D-loop). The gene content, nucleotide composition, and evolutionary rates of each mitogenome were analyzed and compared with those of congeners. A phylogenetic analysis based on 22 mitogenomes in Rana revealed that the two sympatric species were in two different lineages, indicating that they were genetically separated to a certain extent. Three types of gene rearrangement patterns were identified when examining the gene orders of the 22 Rana mitogenomes. Most of the species shared a second and dominant gene rearrangement pattern that originated from the first ancient pattern. A “tandem duplication – multiple deletion” hypothesis was proposed to explain the evolution of these different gene rearrangement patterns. This study provided valuable data references and enhanced our understanding of the phylogenetic implications and gene rearrangements of Rana species.

Addeddate

2025-03-26 14:02:01

Bhl_virtual_titleid

210914

Bhl_virtual_volume

v.1216 (2024)

Call number

10_3897_zookeys_1216_131847

Call-number

10_3897_zookeys_1216_131847

Foldoutcount

0

Genre

article

Identifier

insightsintophy1216liji

Identifier-ark

ark:/13960/s2rm5f271dt

Identifier-bib

10_3897_zookeys_1216_131847

Identifier-doi

10.3897/zookeys.1216.131847

Ocr

tesseract 5.3.0-6-g76ae

Ocr_detected_lang

en

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1.0000

Ocr_detected_script

Latin

Ocr_detected_script_conf

1.0000

Ocr_module_version

0.0.21

Ocr_parameters

-l eng

Page_number_confidence

89

Page_number_module_version

1.0.5

Page_range

63-82

Pages

20

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0.0.25

Possible copyright status

In copyright. Digitized with the permission of the rights holder.

Ppi

300

Source

ZooKeys 1216

Year

2024

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